A total of 150 postpartum (28 to 45 days) apparently healthy buffalo with normal calving history and free from peripartum disorders from college livestock farm and organized dairy farms in and around Jabalpur were screened. After recording history all the animals were subjected to gynaeco-clinical examination, White side test and endometrial cytology by cytobrush technique. On the basis of polymorphonuclear cell (PMN) percentage the animals were divided into different groups of normal, clinical and sub-clinical endometritic buffaloes. Endometrial cytology by cytobrush technique in different groups (normal, clinical and sub-clinical endometritic buffaloes) revealed PMN percentage to be 4.34±1.85, 35.35±3.43 and 21.17±0.45%, respectively. The difference between the groups was signifi cant (p<0.05). Fibroblasts count was recorded as 0.20±0.09 and 12.33±1.61% in subclinical

and clinical endometritic buffaloes while fi broblasts were not observed in endometrial smears of normal buffaloes. The difference was signifi cant (p<0.05) between the groups. It was concluded that endometrial cytology by cytobrush technique was easy and accurate method to diagnose subclinical endometritis in postpartum Murrah buffaloes.